The micro-RNA miR-34a has also been identified in explanted valves as an inhibitor of Notch1 mRNA expression, and administration of an miR-34a inhibitor results in attenuation of aortic valve calcification and stenosis in a murine wire-injury model of CAVD (162). This evidence concerns the gene NOTCH1 and congenital bilateral aplasia of vas deferens from CFTR mutation.