Therefore, in our study, a large number of in vivo and in vitro experiments have been demonstrated that melatonin-mediated MT2 inhibits aeromonas-goblet cell interactions to restore the level of MUC2 production via the LPS/TLR4/MyD88/GSK-3β/ROS/NF-κB loop, further improving colitis in SD mice. The gene discussed is NFKB1; the disease is colitis.