Among them, HT29 and SW480 cells showed the most obvious differential expression, which was consistent with the cell lines with the most significant differential expression of miR-495-3p (Fig. 3G), indicating that HMGB1 was highly expressed in CRC and had a certain negative correlation with the expression of miR-495-3p (Fig. 3H). The gene discussed is HMGB1; the disease is colorectal carcinoma.