To identify the synergistic antitumor effect of si-PD-1 combined with si-TGF-β, H22 cells treated with blank medium, si-NC, si-PD-1, si-TGF-β, and si-PD-1 + si-TGF-β were transplanted into mice to establish the xenograft animal model; when the tumor size reached 100 mm3, RFA was performed. Here, TGFB1 is linked to neoplasm.