Further ROC curve presented that HDAC4 had certain ability of discriminating AIS patients from controls, with an area under curve (AUC) of 0.748 (95% confidence interval (CI): 0.689–0.806); meanwhile, HDAC4 expression was 27.85 at the best cut‐off point (the point with maximum value of the sum of sensitivity and specificity); the sensitivity and specificity were 0.534 and 0.925 at the best cut‐off point, respectively (Figure 1B). Here, HDAC4 is linked to androgen insensitivity syndrome.