TIGIT and neoplasm: This effect was also seen when the analysis was restricted to distant recurrences (Fig. 5b), which additionally showed lower expression of genes or signatures for apoptosis, CD8+ T-cells, IFNγ signaling, stromal cells, T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibition motif domains (TIGIT), and regulatory T cells (Treg), relative to the primary tumor (Fig. 5c–h; Supplementary Table S3).