To fully analyse the strength of MUC1 epitope recognition by obtained MUC1 mouse sera the response to native mucins from pig gastric mucous (Porcine gastric mucin, PGM, Sigma Aldrich) and MUC1 epitope in chimeric NoV VP1-MUC1 expressing L. tarentolae cell lysates was compared to tumour-associated MUC1 peptide serving as a positive control (Fig. 4D). Here, MUC1 is linked to neoplasm.