Moreover, as compared with normal saline, Fe3O4-siNC and Fe3O4-siNC@M-BV2 also significantly inhibited the GPX4 expression, GSH and ROS level in orthotopic drug-resistant GBM tissue, indicating that Fe3O4-siNC and Fe3O4-siNC@M-BV2 induced ferroptosis and subsequently inhibited the growth of orthotopic drug-resistant glioma. The gene discussed is GPX4; the disease is glioblastoma.