Moreover, Fe3O4-siNC@M-BV2, Fe3O4-siNC@M-BV2 + magnet, Fe3O4-siPD-L1@M-BV2 and Fe3O4-siPD-L1@M-BV2 + magnet obviously inhibited Ki67 expression and increased the number of TUNEL positive cells in orthotopic drug-resistant GBM tissue as compared with normal saline, free TMZ and Fe3O4 (Fig. 7E–G). This evidence concerns the gene MKI67 and glioblastoma.