The SIRT1 expression was significantly lower in infiltration (T1 + T2) than that in infiltration (T3 + T4) of ESCC (OR = 0.47, 95% CI: 0.27–0.82), the difference was statistically significant (Z = 2.67, P < 0.05), (Figure 4). This evidence concerns the gene SIRT1 and esophageal squamous cell carcinoma.