The result indicated that SIRT1 expression was lower in ESCC without lymph node metastasis than that with tumor lymph node metastasis (OR = 0.47, 95% CI: 0.31–0.69), and the difference was statistically significant (Z = 3.76, P < 0.05) (Figure 6). The gene discussed is SIRT1; the disease is esophageal squamous cell carcinoma.