Validation of this approach was established by the early-onset detection of the acute-phase immune response proteins, CRP and LBP, which are benchmark biomarkers for human sepsis;7,8,18 early-onset increased abundance of immune modulator proteins CD14 and MPO that stimulate TLR4 and neutrophil activation;29,30 and marked overlap of proteomic changes with recently discovered pathogenic-proteomic-signatures for prediction of SA patient mortality.22 Here, TLR4 is linked to Sepsis.