2012; Chen et al. 2021). As NF-κB signal pathway plays an important role in Bcr-Abl-mediated pathways in CML (Carra et al. 2016), we tested the effects of stachydrine on p-IĸB and p-NF-κB in CML cells after stachydrine treatment. Our results showed that stachydrine at 40 and 80 μM did not affect p-IĸB and p-NF-κB in KCL22 and Ba/F3 T315I cells. Stachydrine at higher concentrations, such as 200 μM and above, did significantly inhibit p-IĸB and p-NF-κB (Figure 7). The gene discussed is ATP8A2; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.