Also, S100P + SPP1− iCCAs significantly correlated with higher CA19-9 (P < 0.01), CEA (P < 0.01), Ki67 expression (P = 0.025), lymph node metastasis (P = 0.013), and advanced TNM stage (P = 0.021), but negatively correlated with tumor size (P = 0.019), HBsAg status (P < 0.01), chronic hepatitis (P = 0.002) and liver cirrhosis (P = 0.049) (Fig. 2f and Supplementary Data 3). Here, SPP1 is linked to cirrhosis of liver.