ALOX5 and acute respiratory distress syndrome: To investigate the contribution of ferroptotic cell death in CCl4-induced ALI, we performed real-time PCR analysis of putative molecular markers of ferroptosis and found that CCl4 treatment robustly induced increased mRNA levels of liver prostaglandin-endoperoxide synthase 2 (Ptgs2), 15-LOX, 12-LOX, and 5-LOX (Fig. 1B).