It is possible that the robust Evx1 expression observed by ICC is due to those Evx1+ surviving cells expressing relatively higher levels of Evx1 compared to others that succumbed to puro, and that the discrepancy between ICC and flow data is due in part to this relatively higher expression as well as the transient expression of Evx1 known to occur in these cells [11]. Here, EVX1 is linked to intrahepatic cholangiocarcinoma.