We found that the densities of NK cells, B cells, Th17 cells, CD3+CD8+EOMES+PD-1− T cells, and CD3+CD8+EOMES−PD-1+ T cells, respectively, were significantly decreased in the peripheries compared to the tumor areas in DCC, but not in ICC (Additional file 1: Figs. S5, S6). The gene discussed is DCC; the disease is intrahepatic cholangiocarcinoma.