And increasing DNA damage in HCC cells with WIP1 knockdown or inhibition was found via comet assay (Fig. 4E, F, and Additional file 1: Fig. 4A), which was further confirmed via immunofluorescence staining of γH2AX foci in HCC cells with WIP1 knockdown or inhibition (Fig. 4G, H, and Additional file 1: Fig. 4B-C). The gene discussed is PPM1D; the disease is hepatocellular carcinoma.