To investigate whether the promoter methylation may serve as a regulator of the transcription of SNCA in BLCA, we treated the BLCA cell lines with 5-Aza-CdR, a demethylating agent approved by the U.S. Food and Drug Administration, at a final concentration of 5 μM for 72 h. This evidence concerns the gene SNCA and bladder transitional cell carcinoma.