Nec-1, RIP3, inhibitor has a therapeutic effect on colitis mice via promoting ZO-1 expression and the proportion of Treg cells, and alleviating the secretion of inflammatory cytokines and necroptosis of mouse intestinal epithelial cells by inhibiting TLR4/MyD88/NF-κB and ROS generation. The gene discussed is MYD88; the disease is colitis.