According to the results from stage one and stage two, we confirmed that higher methylation levels of 17 targets in AMH, C17orf82, HDAC9, IGFBP3, LRRC10B, PDE3A, PRDM6, SYT7 and TBX2 genes were associated with a lower risk of ischemic stroke. Here, HDAC9 is linked to ischemic stroke.