The results showed that the mean methylation levels of AMH, C17orf82, HDAC9, IGFBP3, LRRC10B, PDE3A, PRDM6, SYT7 and TBX2 were significantly (Wilcoxon's two sample test) lower in ischemic stroke cases than in controls (Supplementary Figure 1). This evidence concerns the gene PDE3A and ischemic stroke.