In this study, we (I) investigated the effects of glioma growth on gut microbiome in mice, (II) studied the effects of gut microbiome dysbiosis on glioma development and the expression of CD8 and Foxp3 in tumor microenvironment, and (III) evaluated the role of a balanced gut microbiome microenvironment in glioma development. Here, CD8A is linked to central nervous system cancer.