Here, we observed that GPR65 expression level was upregulated in inflamed gut mucosa and peripheral blood (PB)‐CD4+ T cells of active IBD patients by contrast with healthy controls (HC), and found that transduction with lentivirus (LV) expressing GPR65 (LV‐GPR65) promoted the differentiation of PB‐CD4+ T cells into Th1 and Th17 cells. Here, CD4 is linked to inflammatory bowel disease.