To further verify the targeted binding relationship between miR-23a-3p and Runx2 and its role in oral squamous cell carcinoma, we simultaneously transfected miR-23a-3p mimics and Runx2 overexpression plasmids and detected the changes in cells by CCK-8, Transwell, and clone formation assay. Here, RUNX2 is linked to oral cavity squamous cell carcinoma.