TGFB1 and neoplasm: Given that stromal myofibroblasts can suppress immune microenvironment by TGF-β production 25, and TGF-β can promote angiogenesis, metastasis, tumor cell EMT and fibroblast activation 40, we further evaluated the stromal-related signature enrichment using gene sets (angiogenesis, EMT2, EMT3 and pan-fibroblast TGF-β included) derived from Mariathasan et al. 40, and then confirmed their activation in FAC3 tumors (Figure S5D).