In summary, this study suggests the miRNAs including miR-22-3p, miR-154-5p, miR-34a-5p, miR-485-3p, miR-93-5p, and miR-9-5p and the genes including LEF1, RUNX2, CSF1R, CDKN1A, and FBN1 are the key factors in the progression of osteosarcoma. Here, CDKN1A is linked to osteosarcoma.