We observed that the four treatment schedules with anti-CTLA-4 induced significant decrease of tumor growth starting from day 17 with the treatment at days 3, 6, 9, at days 3, 6, 9, 12, and at days 6, 9, 12 whereas starting from day 22 for treatment at days 9, 12, 15, 18 was not effective (Fig. S1A). This evidence concerns the gene CTLA4 and neoplasm.