Similar to the in vivo evidence, H/R-induced mitochondrial apoptosis and apoptotic cascade exhibited a marked reduction after AdKDM3A infection but induced the opposite effect in KDM3A-knockdown cells, as indicated by flow cytometry for the apoptotic rate (Fig. 6e), ATP content (Fig. 6f) and the protein detection of cytoplasmic/mitochondrial cytochrome c, Bax, Bcl-2 and cleaved caspase-9/3 (Fig. 6g–j). Here, CASP9 is linked to infection.