In contrast, VAF was unstable between primary AML and PDX cells in the following gene mutations: FLT3-ITD (r2 = 0.149, P = 0.017), FLT3-TKD (r2 = 0.0824, P = 0.42), WT1 (r2 = 0.039, P = 0.48) CEBPA (r2 = 0.0377, P = 0.75), NRAS (r2 = 0.001, P = 0.91), KRAS (r2 = 0.0249, P = 0.77), and DNMT3A (r2 = 0.3571, P = 0.009). Here, DNMT3A is linked to acute myeloid leukemia.