We further explored whether FBXO31 is involved in STYX-mediated biological effects of GC cells and found that the biological behaviors of GC cells mediated by STYX overexpression alone can be reversed by the co-expression of STYX and FBXO31, indicating FBXO31 may serve as a critical target for STYX in GC cells. This evidence concerns the gene STYX and gastric cancer.