CD4 and neoplasm: We developed this further by focusing on CD25, because (1) CD25 was upregulated on Treg (relative to conventional CD4 and CD8), at the same time point of the Tocky analysis (figure 2D–F), and (2) CD25 has been shown to be an effective target for Treg depletion.14 Indeed, we found that by adding an optimally depleting anti-CD25 antibody to HSV/BRAFi, we were able to cure 100% of mice, which were subsequently protected from tumor rechallenge (figure 5).