Although Mcl-1 was a predicted target of miR-491-5p and miR-342-5p, neither miRNA significantly modulated its protein expression in osteosarcoma cells, with a maximum decrease of 1.2-fold for miR-342-5p in HOS cells and for miR-491-5p in SaOS-2 cells (Figure 4E). Here, MCL1 is linked to osteosarcoma.