We utilized a rat model of myocardial infarction (MI) and applied CR after heart failure development (4 weeks after MI), with or without application of the AMPK inhibitor compound C. In addition, the role of AMPK in mediating the CR effects in postischemic myocardium was also investigated in alpha 2 AMPK knock-out (KO) mice. The gene discussed is PRKAA2; the disease is heart failure.