CXCR4 and fibrosis: Collagen reduction was also verified by the expression of Col1a1 expression using RT-PCR, as shown in Figure 7C. Overall, the morphological and mRNA assessments indicate that treatment with Chol-PCX/anti-miR-155 particles results in the efficient silencing of miR-155 and CXCR4 antagonism in the AALD fibrosis livers, which is likely due to reduced KC-mediated signaling and HSC activation.