In the present study, we focused on the formulation of thermosensitive Cs-hydrogel cross-linked by β-glycerophosphate (β-GP) alone or in combination with 10% Pluronic F127 (PL F127) for localized injection of 5-FU in breast cancer tissues, which to the best of our knowledge, has not been investigated in breast cancer management via loading and controlled release of 5-FU for more than one month. Here, PPIB is linked to breast cancer.