In the lung tissues of the OVA-and-PM10-treated models, there were significant pathological histological changes, such as thickening of epithelial cells in the airways and lungs, metaplasia of mucin-secreting goblet cells and excessive collagen deposition by AEO nebulization for 5 min/day for a total of 44 days, indicating that AEO has a property to prevent airway structural changes through the trachea to the lung in asthma. The gene discussed is MUC5AC; the disease is asthma.