In addition, dynamic in vitro studies using THP-1 cell-based inflammation with anti-TNF-α mAb-CS/PAMAM dendrimer NP-loaded hydrogel showed that the hydrogel maintained a high therapeutic concentration over a longer period and abruptly decreased the level of free TNF-α on day 14, thus demonstrating their promising therapeutic potential in arthritis treatment [79,166]. This evidence concerns the gene TNF and arthritic joint disease.