Hereby, the aim of the study was to determine inflammatory and regulatory cytokines IL-1α, IL-4, IL-6, IL-12, IL-13, IL-17A, TNF-α, and IFN-γ in the milk of dairy cows with subclinical and clinical mastitis, and to differentiate the changes of their expression throughout three consecutive days after the settlement of the diagnosis of subclinical or clinical mastitis. Here, TNF is linked to mastitis.