TNF and mastitis: For cows with subclinical mastitis, a one-way repeated-measures ANOVA determined no statistically significant difference of mean immunoreactive cell count numbers (%) over time for all factors IL-1α (p = 0.294), IL-4 (p = 0.085), IL-6 (p = 0.081), IL-12 (p = 0.494), IL-13 (p = 0.147), IL-17A (p = 0.084), TNF-α (p = 0.294), and IFN-γ (p = 0.156).