To complement this study, and firmly establish the role of LXRα in the enhanced hepatic steatosis seen in HMGB1ΔHep mice, LXRα expression was knocked down in vivo using an adenovirus expressing short hairpin RNA (shRNA) targeting the receptor (Ad-ShLxrα) (Fig. 5B and fig. This evidence concerns the gene NR1H3 and fatty liver disease.