In addition, hepatic lipid accumulation in AAV8-HMGB1 mice was more reduced compared to control AAV8-eGFP mice (Fig. 2, C to E), as supported by a decrease of Oil Red O staining on liver sections (Fig. 2C), a decrease of hepatic lipid content (Fig. 2D), and a down-regulation in gene expression of liver steatosis canonical markers (Cd36, Cidec, or Adrp47) (Fig. 2E). The gene discussed is CD36; the disease is fatty liver disease.