Degranulation was a prerequisite for perforin-granzyme-mediated tumor-killing of CAR-T cells, CD5/CD7 bispecific CAR-T cells upregulated CD107a expression after co-incubated with Jurkat, CCRF-CEM, MOLT-4, SUP-T1, CCRF-CD5KO cells, and CCRF-CD7KO cells. The gene discussed is CD7; the disease is neoplasm.