Moreover, the V+61+E+ population at E10 to E11 co-expresses surface markers associated with HE/HSC potential, including CD133 (Prominin 1), AA4.1, ESAM, and CD105 (Endoglin), specifically enriches for expression of arterial markers CD44 and DLL4 (both recently associated with pre-HSC/HSC activity in the AGM24,28,30,41) within the AGM VE-Cadherin+ EC population, and heterogeneously express hematopoietic-specific markers CD41, CD43, CD45, and c-kit (Supplementary Fig. 2d). The gene discussed is ESAM; the disease is hereditary elliptocytosis.