Furthermore, “HSC precursors” comprise a comparable number of cells in the V+61+E+ scRNA-seq data to that predicted based on HSC CFC determined functionally by single-cell index and co-culture analysis at E10 and E11 (Fig. 3h, Supplementary Fig. 4e)14 and include a transcriptionally defined subset of HE that lack expression of hematopoietic markers (Itga2b/CD41, Spn/CD43, and Ptprc/CD45) (Fig. 3h). The gene discussed is SPN; the disease is hereditary elliptocytosis.