Among the six sites where IPTi showed measurable efficacy were Ashanti, Ghana, where more than 60% of baseline infections had four or more dhfr/dhps mutations [85]; Tamale, Ghana, where the dhfr triple mutant plus dhps A437G (i.e., the quadruple mutant) was found in 44% of infected children aged less than five years [86]; and Manhica, Mozambique, where prevalence of the dhfr/dhps quintuple mutant in the placebo arm was 44% [87]. This evidence concerns the gene DHFR and infection.