Additionally, in these datasets, we depicted differences in the molecular alterations specific for each histotype: in LUAD, the most altered genes (except TP53) were KRAS (36%), CDKN2A (24%), KEAP (19%), STK11 (19%), EGFR (17%), NF1 (13%), and BRAF (11%); in the LUSC dataset, the most altered genes were PIK3CA (60%), CDKN2A (45%), PTEN (18%), NF1 (15%), and EGFR (10%); in SCLC, RB1 (73%) and NOTCH1 (13%) were the most mutated cancer genes (Figure 2). This evidence concerns the gene RB1 and cancer.