In fact, taking advantage of the use of 3D spheroid cultures, we also reported that this method may be suitable for the screening of several anti-glaucoma drugs toward TGF-β2 and dexamethasone-treated 3D HTM spheroids as in vitro models for HTM with primary open-angle glaucoma and steroid-induced glaucoma [20,21,24]. Here, TGFB2 is linked to open-angle glaucoma.