ACTA1 and occult macular dystrophy: To elucidate the underlying mechanisms responsible for the above effects by PGF2α and OMD on the TGF-β2-treated 2D- and 3D-cultured HTM cells, the expression of ECM proteins (COL1, 4, and 6, FN, and αSMA) was estimated by qPCR analysis and immunolabeling.