CNR1 and prostate carcinoma: To examine the next step in the CBR intracellular signaling cascade after G protein activation in prostate cancer cells, modulation of the activity of the downstream enzyme adenylyl cyclase was evaluated by quantifying intracellular forskolin-stimulated cAMP levels in the presence of a receptor-saturating concentration (10 μM) of high-affinity CBR ligands (Figure 5B).