PIGG and infection: Liu et.al used the FLAER-specific staining method and proved that the deletion of GPI7 caused the significant reduction of GPI-anchored proteins on the plasma or cell wall region of conidia, hyphae, appressorium, and invasive hyphae in M. oryzae, and found that the ΔCgGPI7 mutants were easily recognized by the host immune system because the defect GPI anchor proteins caused the exposure of cell wall chitin and β-1,3-glucans during the infection process, illuminating the GPI anchoring-mediated pathogen–host interaction using the modal M. oryze-Rice system [12].