Because limited research has been conducted on the effect of dietary nitrate/nitrite on NASH with cardiovascular involvement, we attempted to evaluate the effects of dietary nitrite supplementation on NASH pathology in this rat model to determine the molecular mechanism underlying the action of nitrite, particularly compared with that of the ACE inhibitor, which has been demonstrated to have a preventive effect on the development of NASH pathology [10]. The gene discussed is ACE; the disease is metabolic dysfunction-associated steatohepatitis.