To further verify regulatory relationship in these reciprocal miRNA-mRNA pairings, EA and AA PCa cell lines were transfected with miR-34a-5p mimic, miR-99b-5p mimic or miR-96-5p antagomir then followed by RT-qPCR and Western blot analysis to examine the expression of the predicted miRNA target genes (miR-34a-5p targets genes PIK3CB, HIF1A, and IGFBP2; miR-99b-5p target gene MTOR; and miR-96-5p target gene MAPKAPK2) at mRNA and protein levels. The gene discussed is MTOR; the disease is posterior cortical atrophy.