To further verify that COL11A1 promoted the EMT process in pancreatic cancer cells by activating the AKT/GSK-3β/Snail signaling pathway, we first detected the expression levels of E-cad, N-cad, VIM, and MMP-2/9 in PANC-1 cells with different treatments by Western blotting. This evidence concerns the gene GSK3B and familial pancreatic carcinoma.