-Decreased the HDAC activity in a dose-dependent manner-Decreased the protein levels of HDAC1, HDAC2, HDAC3, DNMT1, and DNMT3A-Downregulated the levels of DNMT3A, HDAC1, HDAC6, SET domain proteins (SETD1A, D4, D6), and lysine specific demethylases (KDM 5B, 5C, 4A)⇒ Silibinin inhibits specific histone deacetylases, histone demethylases and DNA methyltransferases, which might cooperatively contribute to the anti-cancer efficacy of this non-toxic phytochemical. This evidence concerns the gene HDAC2 and cancer.