Reduced miR-21 promoter activity and expression in a dose-dependent manner by inhibiting AP-1 binding to the promoterInduced the expression of the tumor suppressor Pdcd4 (programmed cell death protein 4), which is a target of miR-21Arrested the Rko and HCT116 cells in the G2/M phase with increasing concentrationsInhibited the tumor growth, invasion and in vivo metastasis in the chicken-embryo-metastasis assayInhibited the miR-21 expression in primary tumors generated in vivo by Rko and HCT116 cells. This evidence concerns the gene JUN and neoplasm.