-Inhibited the cell proliferation (10–30%) at the highest concentration (250 μM)-Induced apoptotic and necrotic cell death (250 μM)-Induced the DNA double strand breaks (DSBs) and micronuclei production-Increased the frequency of p53 binding protein (53BP1) foci⇒ Diosmin may induce DNA andchromosomal damage in DU145 prostate cancer cells, which, in turn, may provoke apoptotic cell death and may have implications for diosmin-based anticancer therapy. Here, TP53BP1 is linked to prostate cancer.