Demethylated the first 14 CpG sites of the CpG island of the Neurog1 geneRestored the expression of this cancer-related CpG-methylation epigenome marker genePossessed limited effects on the expression of epigenetic modifying proteins MBD2, MeCP2, DNMT1, and DNMT3aDecreased the MeCP2 binding to the promoter of Neurog1Induced different effects on the protein expression of HDACsIncreased the expression of HDAC1, 4, 5, and 8 but decreased the HDAC3Decreased the total HDAC activityDecreased the enrichment of H3K27me3 at the Neurog1 promoter region as well as at the global level. The gene discussed is MBD2; the disease is cancer.