Induced the DLC1 expression by demethylation of DLC1 promoter in MDA-MB- 361 cellsInhibited the proliferation of MDA-MB-361 cells via DLC1Inhibited the DNMT1 expressionInhibited the Sp1 expression to regulate DNMT1 and DLC1Inhibited the breast cancer growth in vivo. This evidence concerns the gene DLC1 and breast cancer.