-Suppressed PRC2 (Polycomb Repressive Complex 2) expression-Reduced the EZH2 (Enhancer of Zeste Homolog 2) expression while increasing trimethylation levels of Lys27 on H3 (H3K27me3)-Induced a modest concentration-dependent increase in DNMT activity-Induced a concentration-dependent decrease in HDAC1-2 expression levels⇒ Silibinin induces epigenetic alterations in human prostate cancer cells. The gene discussed is DNMT1; the disease is prostate cancer.