We isolated EVs from the culture media of different metastatic melanoma cell lines (mel P, mel H, and mel Kor) and showed by DLS that their diameter is about 50–200 nm, which is in accordance with the previously published data [42,43]; they are coated by the lipid membrane and express the exosomal markers CD63 and TSG101 (Supplementary Figures S1 and S2). This evidence concerns the gene TSG101 and melanoma.