In addition, results from growth and migration assays (Figure S3B) demonstrated that elevated HSF1 resulted in a more disorganised growth pattern in 3D culture, and that cells exhibited significantly more migratory behaviour with higher HSF1 (WT *** p < 0.001, RDT *** p < 0.001), both characteristics of a more metastatic cancer phenotype. Here, HSF1 is linked to cancer.